Jessie is an excitable, rough-and-tumble cowgirl doll who's a popular character in the Toy Story series. She's one of the few female characters in the series and has become a beloved character for fans worldwide.

She is a major character in Toy Story 2 and Toy Story 3. She plays an important role in Woody and Buzz's rescue of Bonnie's toys from Sunnyside Daycare, though she initially resists their plan to leave Andy's house and start a new life at the daycare.

After lotso and his minions reset Buzz into a Spanish version of himself, he is shown to be more attracted to Jessie than he has ever been before, calling her "my desert flower" and dancing around her; she shields him from Mr. Potato Head; she tries to kiss him, but he turns her down. She also saves him from being pinned under the trash and from a falling television set, making him realize that she loves him.

She is a strong, loyal, and spirited character who will do anything for her friends. She's the leader of the group and is often the only one who sees the good in her friends. She is also known for her ability to overcome a fear of claustrophobia, a phobia that she confronts in Toy Story of Terror!